Journal: PLOS ONE
Article Title: (−)-Epicatechin regulates endoplasmic reticulum stress and promotes ferroptosis in lung cancer cells via the PERK/eIF2α/ATF4 signaling pathway
doi: 10.1371/journal.pone.0313010
Figure Lengend Snippet: A: Western blotting was used to detect the expression levels of PERK/eIF2α/ATF4 signaling pathway-related proteins and CHOP in lung cancer cells. B: Flow cytometry was used to measure the Ca 2+ levels in lung cancer cells. C: CCK-8 detection of lung cancer cell viability. D: Detection of Fe 2+ content in lung cancer cells using a reagent kit. E: Western blotting was used to detect the expression levels of the ferroptosis-related proteins SLC7A11, GPX4, and FTH1 in lung cancer cells. ** P<0.01, * * * P<0.001 vs. NC# P<0.05, # # P<0.01, # # # P<0001 vs. EC.
Article Snippet: Primary antibodies against SLC7A11 (1:2000, ab175186, Abcam, UK), GPX4 (1:1000, ab125066, Abcam, UK), FTH1 (1:1000, PA5-27500, Thermo Fisher Scientific, USA), p-PERK (1:1000, PA5-40294, Thermo Fisher Scientific, USA), PERK (1:1000, PA5-99447, Thermo Fisher Scientific, USA), p-eIF2α (1:1000, MA5-15133, Thermo Fisher Scientific, USA), eIF2α (1:1000, AHO0802, Thermo Fisher Scientific, USA), ATF4 (1:1000, ab1371, Abcam, UK), CHOP (1:1000, MA1-250, Thermo Fisher Scientific, USA), and β-actin (1:1000, ab8226, Abcam, UK) were added overnight at 4 °C.
Techniques: Western Blot, Expressing, Flow Cytometry, CCK-8 Assay